RBS
Part:BBa_K2619012:Design
Designed by: shiyu liu Group: iGEM18_XJTLU-CHINA (2018-09-07)
internal ribosome entry site/IRES from Cloning vector pPKm-244
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
no specific considerations, but adding the same IRES will increase the difficulty in gene synthesis, therefore used only one IRES in our final booster
Source
Cloning vector pPKm-244
References
Kyriakakis P, Catanho M, Hoffner N, et al. Biosynthesis of Orthogonal Molecules Using Ferredoxin and Ferredoxin-NADP+ Reductase Systems Enables Genetically Encoded PhyB Optogenetics. ACS Synthetic Biology. 2018;7(2):706-717. doi:10.1021/acssynbio.7b00413.