RBS

Part:BBa_K2619012:Design

Designed by: shiyu liu   Group: iGEM18_XJTLU-CHINA   (2018-09-07)


internal ribosome entry site/IRES from Cloning vector pPKm-244


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

no specific considerations, but adding the same IRES will increase the difficulty in gene synthesis, therefore used only one IRES in our final booster


Source

Cloning vector pPKm-244


References

Kyriakakis P, Catanho M, Hoffner N, et al. Biosynthesis of Orthogonal Molecules Using Ferredoxin and Ferredoxin-NADP+ Reductase Systems Enables Genetically Encoded PhyB Optogenetics. ACS Synthetic Biology. 2018;7(2):706-717. doi:10.1021/acssynbio.7b00413.